Mohammad Reza Sobhan; Mahmood Farshchian; Ali Hoseinzadeh; Hamid Reza Ghasemibasir; Ghasem Solgi
Volume 13, Issue 4 , December 2016, , Pages 317-323
Abstract
Background: As a chronic inflammatory condition, psoriasis results from an interaction
between genetic and immunologic factors in a predisposing environment. In spite of
compelling evidence for the role of T cells and cytokines in psoriasis, interleukin (IL)-
10 and IL-22 have not been sufficiently ...
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Background: As a chronic inflammatory condition, psoriasis results from an interaction
between genetic and immunologic factors in a predisposing environment. In spite of
compelling evidence for the role of T cells and cytokines in psoriasis, interleukin (IL)-
10 and IL-22 have not been sufficiently investigated. Objective: To assess the serum
levels of IL-10 and IL-22 in patients with psoriasis compared to healthy controls.
Methods: A total of 28 patients with psoriasis were compared with 28 age and sexmatched
healthy subjects. Psoriasis Area and Severity Index (PASI) criteria were used
to measure the severity of the disease. Serum levels of IL-10 and IL-22 were measured
in both groups and compared. Results: The mean serum level of IL-10 was 89.5±18.7 in
patients compared to 117.2±23.4 pg/ml in the controls (p=0.36). Also, serum level of
IL-22 was 284.1±49.7 in patients versus 425.4±82.8 pg/ml in control group (p=0.17).
There was a significant direct correlation between levels of IL-10 and IL-22 in patients
group (p=0.0005). The clinical severity of psoriasis was significantly correlated with
high levels of IL-22 (p<0.0001).Conclusions: The decreased levels of IL-10 in psoriatic
patients and direct correlation between higher levels of IL-22 and disease severity
support the clinical implication of both cytokines in psoriasis.
Maryam Hamidinia; Mehri Ghafourian Boroujernia; Abdolhassan Talaiezadeh; Ghasem Solgi; Maryam Taghdiri; Ali Khodadadi
Volume 10, Issue 1 , March 2013, , Pages 22-30
Abstract
Background: Regulatory T cells (T-regs) have an important role in cancer by suppression of protective antitumor immune responses. Regulatory T cells express the forkhead/winged helix transcription factor (FOXP3) and OX40 molecules which have important regulatory roles in the immune system. Objective: ...
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Background: Regulatory T cells (T-regs) have an important role in cancer by suppression of protective antitumor immune responses. Regulatory T cells express the forkhead/winged helix transcription factor (FOXP3) and OX40 molecules which have important regulatory roles in the immune system. Objective: To evaluate FOXP3 and OX40 transcripts in the peripheral blood mononuclear cells of women with breast cancer. Methods: Blood samples from 40 women with histologically-confirmed infiltrating ductal carcinoma of the breast and 40 healthy volunteer women without a history of malignancy or autoimmune disorders were collected. The abundance of FOXP3 and OX40 gene transcripts were determined by quantitative real-time PCR (qRT-PCR). Results: There was a significant positive correlation between FOXP3 and OX40 gene expression in women with breast cancer in a stage dependent manner. Conclusion: This finding emphasizes the importance of T-regs as predominant targets for breast cancer immunotherapy.
Ghasem Solgi; Gholamreza Pourmand; Abdorasool Mehrsai; Mohsen Tahei-mahmoudi; Mohammad Hossein Nicknam; Mohammad Ebrahimi Rad; Ali Seraji; Amirabbas Asadpoor; Bita Ansaripor; Behrouz Nikbin; Aliakbar Amirzargar
Volume 7, Issue 1 , March 2010, , Pages 18-29
Abstract
Background: Anti-HLA-antibodies are known to affect the allograft survival in transplant recipient patients. Objective: The aim of this study was to evaluate the association between anti-HLA antibodies and kidney allograft outcomes, particularly in recipients with concur-rent donor bone marrow cell infusion ...
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Background: Anti-HLA-antibodies are known to affect the allograft survival in transplant recipient patients. Objective: The aim of this study was to evaluate the association between anti-HLA antibodies and kidney allograft outcomes, particularly in recipients with concur-rent donor bone marrow cell infusion (DBMI). Methods: Between June 2006 and May 2007, forty living unrelated donor kidney transplants consisting of 20 recipients with DBMI and 20 without infusion entered into the study and were monitored prospectively for one year. Pre- and post-transplant (days 14, 30, and 90) sera were screened for the presence of anti-HLA class-I and II antibodies, and subsequently positive sera retested with ELISA spe-cific panel for antibody specification. Results: Of 40 patients, 9 (22.5%) experienced acute rejection episodes (ARE) (6/20 cases in non-infused versus 3/20 in DBMI patients). The prevalence of anti-HLA antibodies before and after transplantation were higher in patients with ARE compared to non-rejecting ones (88.8% vs. 38.7%, p=0.01 and 66.6% vs. 25.8%, p=0.04, respectively). A total of 10% (4/40) of patients developed donor specific anti-HLA antibodies (DSA) and in this regard 2 patients from the control group experienced ARE. All 3 rejecting patients in DBMI group were negative for DSA and positive for non-DSA. The lower titer of post-transplant anti-HLA antibodies were shown in DBMI patients compared to pre-transplantation titer. Additionally, the average serum creatinine levels during one year follow up and even in those patients with ARE were lower compared to controls. Con-clusion: Our findings reveal an association between pre- and post-transplant anti-HLA an-tibodies, and ARE and also early allograft dysfunction. It suggests that lower incidence of ARE, undetectable DSA, lower titer of antibodies concomitant with a decrease in serum creatinine level, better allograft function and lower percentages of PRA in DBMI patients, could be the probable manifestations of partial hypo-responsiveness against allografts.
